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[Fungal aspartic proteinase from Trichoderma viride. Specificity during oligopeptide hydrolysis]
A N Simankova1, O A Mirgorodskaia, N V Savel'eva
1Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia.
Bioorganicheskaia Khimiia
|March 18, 1999
Abstract:
We isolated, purified, and characterized an aspartic protease from fungus Trichoderma viride. The pH-dependence of the enzyme functioning was determined, and its specificity in the limited proteolysis of insulin and melittin was compared to the specificities of pepsin A and gastricsin. The kinetics of melittin hydrolysis by these enzymes was studied by mass spectrometry.