Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enzyme-mononucleotide interactions: three different folds share common structural elements for ATP recognition.

K A Denessiouk1, J V Lehtonen, M S Johnson

  • 1Department of Biochemistry and Pharmacy, Abo Akademi University, Turku, Finland.

Protein Science : a Publication of the Protein Society
|March 19, 1999
PubMed
Summary

Three unrelated ATP-dependent enzymes share a common ATP-binding site organization. This convergent evolution highlights how different protein folds can achieve similar local structures for essential functions like ATP binding.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Why methane surged in the atmosphere during the early 2020s.

Science (New York, N.Y.)·2026
Same author

The <sup>40</sup>Ar(d,p)<sup>41</sup>Ar cross section between 3-7 MeV.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2022
Same author

NASA GEOS Composition Forecast Modeling System GEOS-CF v1.0: Stratospheric Composition.

Journal of advances in modeling earth systems·2022
Same author

Interviewing alleged victims with mild and moderate intellectual disabilities and autism: A field study of police-investigated cases of physical and sexual abuse in a Norwegian national sample.

Journal of intellectual disability research : JIDR·2020
Same author

Tracking changes in soil organic carbon across the heterogeneous agricultural landscape of the Lower Fraser Valley of British Columbia.

The Science of the total environment·2020
Same author

ADAPTATION AND RULES OF FORM: CHIRALITY AND SHAPE IN PARTULA SUTURALIS.

Evolution; international journal of organic evolution·2017

Area of Science:

  • Biochemistry
  • Structural Biology
  • Molecular Evolution

Background:

  • ATP-dependent enzymes are crucial for numerous cellular processes.
  • Understanding the structural basis of ATP binding is key to enzyme function.
  • Diverse enzymes often exhibit conserved functional mechanisms despite differing folds.

Purpose of the Study:

  • To investigate the structural similarities in ATP-binding sites across unrelated ATP-dependent enzymes.
  • To identify conserved residues and structural motifs involved in ATP binding.
  • To explore the role of convergent evolution in enzyme function.

Main Methods:

  • Comparative structural analysis of ATP-binding sites.
  • Identification of structurally equivalent residues across different enzyme families.

Related Experiment Videos

  • Analysis of hydrogen bonding and hydrophobic interactions within the ATP-binding pocket.

    • Main Results:

    • Three distinct ATP-dependent enzymes (cAMP-dependent protein kinase, D-Ala:D-Ala ligase, and alpha2beta2 ribonucleotide reductase alpha-subunit) exhibit a conserved organization of their ATP-binding sites.
    • A core set of 23 structurally equivalent residues, including beta-sheet strands and a loop, form the common binding motif.
    • Key hydrogen bonds involving four conserved amino acids orient the adenine moiety of ATP, and a conserved lysine residue anchors the alpha-phosphate.

    Conclusions:

    • Convergent evolution has led to similar local structures for ATP binding in unrelated proteins.
    • Nature utilizes conserved structural fragments to achieve functional requirements, such as efficient ATP binding.
    • This study provides insights into the principles of protein structure and function, demonstrating how different evolutionary paths can converge on optimal solutions.