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Structures of primer-template hybrids in arbitrarily primed polymerase chain reaction
K Kawakami1, J Yasuda, T Kayama
1Oncogene Division, National Cancer Center Research Institute, Tokyo, Japan.
Genetic Analysis : Biomolecular Engineering
|March 20, 1999
Abstract:
Nucleotide sequence analysis of arbitrarily primed PCR products from two known regions of human genome revealed that at least six contiguous bases at the 3'-end of a primer of 20 bases, were perfectly matched in the primer-template hybrids.