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Updated: Jul 20, 2026
07:42
Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis
Published on: November 26, 2015
[Androgen binding proteins in human prostate (author's transl)]
Annales D'Endocrinologie
|March 1, 1976
Summary
Researchers identified two binding components in human prostate cytosol: androgen receptors and sex steroid-binding protein (SBP). SBP-testosterone complexes may associate with prostate cells, influencing testosterone uptake and metabolism.
Area of Science:
- Biochemistry
- Endocrinology
- Molecular Biology
Context:
- Hyperplastic human prostate cytosol contains specific steroid-binding proteins.
- Understanding these proteins is crucial for prostate biology and disease research.
Purpose:
- To identify and characterize high-affinity steroid-binding components in human prostate cytosol.
- To investigate the role of sex steroid-binding protein (SBP) in testosterone uptake and metabolism in prostate tissue.
Summary:
- Glycerol gradient centrifugation revealed two binding components in prostate cytosol: androgen receptors (8-10 S) and sex steroid-binding protein (SBP) (4-5 S).
- Calculated cytosol receptor concentration was 50 fmol/mg protein (5,000 sites/cell), while SBP concentration was 8,000 sites/cell.
- Organ culture experiments with 3H-testosterone suggested that the SBP-testosterone complex might associate with prostate cells, impacting testosterone metabolism.
Impact:
- Provides insights into the molecular mechanisms of androgen and steroid binding in the prostate.
- Suggests a potential role for SBP in modulating prostate cell response to testosterone.
- Contributes to the understanding of steroid hormone action in prostate physiology and pathology.
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