Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The DNA methylation paradox.

P A Jones1

  • 1Department of Biochemistry and Molecular Biology, University of Southern, California/Norris Comprehensive Cancer Center and Hospital, Los Angeles 90033-0800, USA. jones_p@froggy.hsc.usc.edu

Trends in Genetics : TIG
|March 24, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comprehensive Lifestyle Improvement Program for Prostate Cancer (CLIPP) is associated with improvement in weight and components of metabolic syndrome in men exposed to androgen deprivation therapy for prostate cancer.

Prostate cancer and prostatic diseases·2021
Same author

DNA methylation in blood from neonatal screening cards and the association with BMI and insulin sensitivity in early childhood.

International journal of obesity (2005)·2017
Same author

Pharmacokinetics and toxicity of the novel oral demethylating agent zebularine in laboratory and tumor bearing dogs.

Veterinary and comparative oncology·2015
Same author

NMDA receptor binding in focal epilepsies.

Journal of neurology, neurosurgery, and psychiatry·2015
Same author

Pulsed-coil magnet systems for applying uniform 10-30 T fields to centimeter-scale targets on Sandia's Z facility.

The Review of scientific instruments·2015
Same author

Development of the quiescent center in maturing embryonic radicles of pea (Pisum sativum L. cv. Alaska).

Planta·2014
Same journal

Beyond housekeeping: snRNA diversity, regulation, and human disease.

Trends in genetics : TIG·2026
Same journal

Rethinking mitochondrial metabolism: Intraindividual variability meets population constraints.

Trends in genetics : TIG·2026
Same journal

A role for epigenetics in rapid adaptation.

Trends in genetics : TIG·2026
Same journal

The myth of asexual fungi.

Trends in genetics : TIG·2026
Same journal

Rethinking molecular evolution through protein language model embeddings.

Trends in genetics : TIG·2026
Same journal

Co-transcriptional splicing: Distinct phases, mutual benefits, and basis for nuclear architecture.

Trends in genetics : TIG·2026
See all related articles

CpG island methylation typically silences genes at promoters. However, methylation within transcribed regions correlates with gene expression, presenting a paradox potentially resolved by transcription-facilitated methylation.

Area of Science:

  • Epigenetics
  • Molecular Biology
  • Genetics

Background:

  • CpG island methylation is generally associated with gene silencing, particularly when located in gene promoters.
  • This silencing mechanism is crucial for processes like X chromosome inactivation.
  • However, methylation in transcribed regions, distant from promoters, shows a contrasting correlation with gene expression.

Purpose of the Study:

  • To investigate the paradoxical correlation between CpG island methylation and gene expression.
  • To explore the mechanisms underlying methylation patterns in different genomic locations.
  • To reconcile the opposing roles of CpG methylation at promoters versus transcribed regions.

Main Methods:

  • Analysis of methylation patterns in various gene contexts (promoters, downstream regions).

Related Experiment Videos

  • Correlation studies between methylation status and gene expression levels in mammalian cells.
  • Hypothesis generation based on observed methylation-gene expression relationships.

    • Main Results:

    • Methylation of CpG islands in promoters correlates with transcriptional inactivity.
    • Methylation of CpG islands downstream of transcription initiation sites does not inhibit gene elongation.
    • A positive correlation between methylation in transcribed regions and gene expression was observed.

    Conclusions:

    • CpG island methylation has distinct effects depending on its genomic location.
    • The observed "methylation paradox" suggests context-dependent roles for DNA methylation.
    • Transcription through CpG islands may facilitate their de novo methylation, resolving the paradox.