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Updated: Oct 24, 2026
Crystallization of Membrane Proteins in Lipidic Mesophases
Published on: March 28, 2011
Purification, crystallization and preliminary x-ray crystallographic analysis of a phospholipase A2 from Daboia
V Chandra1, A Nagpal, A Srinivasan
1Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110029, India.
Abstract:
Phospholipases are esterolytic enzymes which hydrolyze glycerophospholipids. The pharmacological efficiency of phospholipase A2 (PLA2) enzymes is reflected by their specificity towards a tissue or organ. The Russell's viper has been classified into two classes. Class 1 contains Viper russelli russelli, Viper russelli siamensis and Viper russelli formosensis, whereas class 2 contains Daboia russelli pulchella. The sequence identity between the PLA2s from these two classes is 47%. The novel PLA2 from Daboia russelli pulchella has been crystallized using the hanging-drop vapour-diffusion method with ammonium sulfate as precipitating agent. Crystals belong to the orthorhombic space group C2221 with unit-cell parameters a = 77.01, b = 92.29, c = 76.90 A and two molecules in the asymmetric unit. These crystals diffract to about 2. 49 A resolution using a rotating-anode source.
