An atraumatic method to establish human colon carcinoma in long-term culture

Techniques for creation of colon carcinoma epithelial cells lines in long-term culture have been available for years, but these techniques have involved mechanical or enzymatic methods to separate epithelial cells from surrounding tissues. While this practice has been intermittently successful, the effect of these traumatic methods on long-term cellular behavior is unknown. Samples of colon carcinoma from patient volunteers were subjected to serial nonenzymatic disruptions of carcinoma cells from surrounding fibrous tissues. Cells were collected, allowed to proliferate, and then tested for their epithelial characteristics (mucin, vimentin, cytokeratin, colon-specific antigen, carcinoembryonic antigen) by immunohistochemistry and flow cytometry. Growth characteristics were determined by phase-contrast microscopy, multiple passage, and freeze/thaw effects. Tumorigenicity was proven in nude mice. Of 11 initial attempts, three resulted in stable long-term culture lines of cells which are demonstrated to behave similarly to the original tumors from which they were derived. This technique adds another reliable in vitro tool for the study of colon carcinoma.