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Updated: Aug 13, 2026

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis
Published on: January 26, 2015
Comparative biology of Ca2+-dependent exocytosis: implications of kinetic diversity for secretory function
1Dept of Physiology, Faculty of Medicine, University of Tokyo, Hongo, Japan.
Abstract:
The application of caged-Ca2+ compounds to the study of Ca2+-dependent exocytosis has begun to reveal kinetic intermediates in this important process. The time course of exocytosis varies greatly among different cell and vesicle types, even in response to Ca2+ 'jumps' of identical amplitude. The kinetics of the binding of Ca2+ to the putative Ca2+ sensor for exocytosis also vary. Theoretical analysis reveals that the kinetic diversity of exocytotic and Ca2+-binding reactions has distinct roles in determining the probability of exocytosis occurring. It is proposed that both of these reactions are optimized for the secretory function of specific cell types and that the exocytotic reaction includes vesicle translocation in addition to the fusion of vesicles with the plasma membrane.
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