Related Experiment Videos
Urinary nucleosides
1Department of Pharmacology and Toxicology, College of Pharmacy, University of Arizona, Tucson 85721, USA.
Mass Spectrometry Reviews
|March 30, 1999
Summary
Urinary nucleosides show potential as biomarkers for diseases like cancer. However, current analytical methods require further refinement for reliable clinical decision-making based on nucleoside levels.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Clinical Diagnostics
Background:
- Urinary nucleosides are compounds found in human urine, with 57 identified in both healthy and diseased states.
- Their origins and clinical significance have been studied, particularly in relation to various diseases.
- Previous research has explored their potential as indicators of health status.
Purpose of the Study:
- To review the analytical methods, origins, and clinical significance of urinary nucleosides up to 1997.
- To present structural, chromatographic, and mass spectral data for identified urinary nucleosides.
- To compare the advantages and limitations of different analytical techniques for urinary nucleoside analysis.
Main Methods:
- Gas Chromatography (GC)
- High-Performance Liquid Chromatography (HPLC)
- Gas Chromatography-Mass Spectrometry (GC/MS)
- High-Performance Liquid Chromatography-Mass Spectrometry (HPLC/MS)
- Immunoassays
- Sample preparation techniques
Main Results:
- Detailed data for 57 urinary nucleosides, including structures and spectral information, are presented.
- Analysis of 37 individual HPLC fractions using HPLC separation and GC/MS is discussed.
- A comparison of analytical methods highlights their respective strengths and weaknesses.
Conclusions:
- Urinary nucleosides are confirmed as potential biomarkers for cancer and other diseases.
- Analytical methodologies for urinary nucleoside detection need enhancement for clinical application.
- Further improvements in analytical techniques are necessary for reliable clinical decisions based on urinary nucleoside levels.