Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mapping oxidative DNA damage at nucleotide level.

H Rodriguez1, S A Akman

  • 1Biotechnology Division, DNA Technologies Group, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA. henry.rodriguez@nist.gov

Free Radical Research
|March 31, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Analysis and development of the learning curve in robotic colorectal surgery at a specialized center.

Journal of robotic surgery·2026
Same author

The Interstellar Mapping And Acceleration Probe High Energy (IMAP-Hi) Neutral Atom Imager.

Space science reviews·2026
Same author

The Solar Wind Electron (SWE) Instrument for the Interstellar Mapping and Acceleration Probe Mission.

Space science reviews·2026
Same author

Performance assessment of ISO 23036-1:2021 for parasite detection in the seafood industry.

Food and waterborne parasitology·2026
Same author

Simulated use of the water circuit disinfection device on a Mycobacterium chimaera-contaminated LivaNova 3T Heater Cooler Device: results of mycobacterial and bacterial surveillance.

The Journal of hospital infection·2025
Same author

The Compact Dual Ion Composition Experiment (CoDICE) for the IMAP Mission.

Space science reviews·2025
Same journal

Correction.

Free radical research·2026
Same journal

Midpoint potential of the phenoxyl radical/phenol redox couple of phytocannabinoids as a diagnostic tool for analyzing their prooxidant action in the cell.

Free radical research·2026
Same journal

Photoprotective effects of <i>Caulerpa lentillifera</i> extract against UV-induced cellular damage via Nrf2 activation and MMP inhibition.

Free radical research·2026
Same journal

Procontractile influence of ROS, produced by NADPH oxidase, is greater during contraction induced by thromboxane A<sub>2</sub> than α<sub>1</sub>-adrenoceptor activation.

Free radical research·2026
Same journal

Edaravone attenuates acrylamide-induced nephrotoxicity by modulating the endoplasmic reticulum stress pathway in rats.

Free radical research·2026
Same journal

Acidic plasma-activated povidone-iodine induces copper-dependent oxidative death in oral squamous cell carcinoma cells.

Free radical research·2026
See all related articles

Reactive oxygen species (ROS) cause DNA damage, contributing to cancer and aging. New methods enhance detection of this damage in genes like p53 and PGK1, improving our understanding of oxidative stress.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Reactive oxygen species (ROS) are implicated in aging and cancer pathogenesis due to DNA damage.
  • Investigating ROS-induced DNA modifications is crucial for understanding these conditions.

Purpose of the Study:

  • To map oxidative-induced DNA base modifications in human p53 and PGK1 genes in vivo and in vitro.
  • To develop and validate enhanced methods for detecting ROS-mediated DNA damage.

Main Methods:

  • Utilized an oxidative-induced DNA damage mapping version of Ligation-mediated polymerase chain reaction (LMPCR).
  • Exposed human fibroblasts and purified genomic DNA to hydrogen peroxide (H2O2) and various metal ions (Cu(II), Fe(II)/Fe(III), Cr(VI)).
  • Developed a method combining Continuous Elution Electrophoresis (CEE) with LMPCR to enrich target DNA sequences.

Related Experiment Videos

Main Results:

  • In vivo and in vitro ROS-induced DNA damage patterns were similar, with guanine bases being heavily modified.
  • DNA damage patterns varied with metal ions, with Cr(VI) causing unique thymine modifications.
  • The enhanced LMPCR method (CEE + LMPCR) increased signal strength by 24-fold for detecting ROS-induced DNA damage.

Conclusions:

  • DNA damage probability by H2O2 is sequence-dependent, with limited influence from chromatin structure.
  • DNA-metal ion binding domains may accommodate various metal ions.
  • Enhanced sensitivity of target gene-enriched LMPCR allows for mapping DNA damage in cells exposed to non-cytotoxic ROS levels.