Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mucosal dendritic cells and immunodeficiency viruses.

M Pope1

  • 1Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, NY 10021, USA. popem@rockvax.rockefeller.edu

The Journal of Infectious Diseases
|April 1, 1999
PubMed
Summary

Dendritic cells (DCs) and T cells support human immunodeficiency virus type 1 (HIV-1) replication in skin and mucosal tissues. This DC-T cell interaction is crucial for virus growth, regardless of viral subtype or tropism.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rapid Proteomics to Prospect and Validate Novel Bacterial Metabolism Induced by Environmental Burden.

Methods in enzymology·2017
Same author

Developmental programming, adiposity, and reproduction in ruminants.

Theriogenology·2016
Same author

Childhood trauma, midbrain activation and psychotic symptoms in borderline personality disorder.

Translational psychiatry·2015
Same author

Impulsivity in borderline personality disorder.

Psychological medicine·2015
Same author

Effect of backrest inclination on the transmission of vertical vibrations through the lumbar spine.

Clinical biomechanics (Bristol, Avon)·2013
Same author

Test-retest variation in lifting capacity and indices of subject effort.

Clinical biomechanics (Bristol, Avon)·2013

Area of Science:

  • Immunology
  • Virology
  • Cell Biology

Background:

  • Dendritic cells (DCs) play a role in human immunodeficiency virus type 1 (HIV-1) pathogenesis.
  • Skin and mucosal tissues are key sites for HIV-1 infection and transmission.

Purpose of the Study:

  • To investigate the role of dendritic cells (DCs) and T cells in HIV-1 replication.
  • To determine if DC-T cell interactions support virus growth in a skin-mucosal model.

Main Methods:

  • Utilized skin explants as a model for mucosal tissues.
  • Co-cultured dendritic cells (DCs) and T cells from human and macaque sources.
  • Assessed HIV-1 and simian immunodeficiency virus (SIV) replication in these co-cultures.

Main Results:

  • HIV-1 replicated efficiently in the DC-T cell milieu of skin explants, forming syncytia.
  • Virus replication occurred irrespective of HIV-1 subtype, syncytium-inducing capacity, or tropism.
  • Similar DC-T cell interactions supported simian immunodeficiency virus (SIV) replication in macaques.
  • Active virus replication in both human and monkey systems required the presence of both DCs and T cells.

Conclusions:

  • Dendritic cells (DCs) and T cells are essential for HIV-1 replication in skin and mucosal tissues.
  • DC-T cell syncytia are a site of active HIV-1 replication.
  • Further research using the macaque model will explore DC roles in HIV transmission and spread.

Related Experiment Videos