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Apolipoprotein E in cerebrospinal fluid: relation to phenotype and plasma apolipoprotein E concentrations
K Yamauchi1, M Tozuka, T Nakabayashi
1Central Clinical Laboratories, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto 390-8621, Japan.
Clinical Chemistry
|April 2, 1999
Summary
Cerebrospinal fluid (CSF) apolipoprotein E (apoE) levels are higher in younger individuals and correlate with CSF lipids. These findings suggest apoE and lipids in CSF may originate from within the central nervous system.
Area of Science:
- Neuroscience
- Biochemistry
- Clinical Chemistry
Background:
- Apolipoprotein E (apoE) is implicated in Alzheimer's disease pathogenesis.
- Limited data exist on apoE concentrations in cerebrospinal fluid (CSF).
Purpose of the Study:
- To measure apoE concentrations in CSF.
- To correlate CSF apoE levels with apoE phenotype and CSF lipids.
- To investigate the origin of CSF apoE and lipids.
Main Methods:
- Developed a sensitive ELISA assay for CSF apoE measurement.
- Analyzed CSF apoE, total cholesterol, and phospholipid levels.
- Correlated CSF analyte concentrations with age, sex, apoE phenotype, and plasma levels.
Main Results:
- CSF apoE concentrations were significantly higher in individuals aged 0-5 years compared to older individuals.
- CSF apoE levels correlated positively with CSF total cholesterol and phospholipids.
- CSF apoE, total cholesterol, and phospholipid concentrations did not correlate with plasma levels.
Conclusions:
- CSF apoE and lipid concentrations may reflect local production within the central nervous system.
- Findings suggest limited transfer of apoE and lipids across the blood-brain barrier.