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Semiautomated metabolic staining assay for Bacillus cereus emetic toxin
W J Finlay1, N A Logan, A D Sutherland
1School of Biological and Biomedical Sciences, Glasgow Caledonian University, Glasgow G4 OBA, United Kingdom.
Abstract:
This paper describes a specific, sensitive, semiautomated, and quantitative Hep-2 cell culture-based 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay for Bacillus cereus emetic toxin. Of nine Bacillus, Brevibacillus, and Paenibacillus species assessed for emetic toxin production, only B. cereus was cytotoxic.
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