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Related Experiment Videos

Bone marrow processing on the Haemonetics V50 cell separator.

N A Anderson1, J M Cornish, V Godwin

  • 1South Western Regional Transfusion Centre, Bristol, U.K.

Transfusion Science
|December 10, 1989
PubMed
Summary
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This study details a bone marrow processing method using the Haemonetics V50 cell separator, achieving high mononuclear cell recovery. The buffy coat fraction is suitable for cryopreservation and purging, ensuring adequate stem cell retention for transplantation.

Area of Science:

  • Hematology
  • Cellular Therapy
  • Transplantation Medicine

Background:

  • Bone marrow (BM) processing is crucial for stem cell transplantation.
  • Efficient isolation of mononuclear cells (MNCs) is vital for successful engraftment.
  • Methods for reducing contaminating erythrocytes in BM harvests are needed, especially in ABO-incompatible transplants.

Purpose of the Study:

  • To evaluate the efficacy of the Haemonetics V50 cell separator for processing bone marrow harvests.
  • To assess the recovery and quality of mononuclear cells (MNCs) in the buffy coat (BC) fraction.
  • To determine the effectiveness of the procedure in reducing donor erythrocytes in ABO-incompatible scenarios.

Main Methods:

  • Processing of 27 bone marrow harvests using the Haemonetics V50 with a pediatric plasmapheresis set.

Related Experiment Videos

  • Collection of buffy coat (BC) fraction, with subsequent assessment of MNC count and recovery.
  • Evaluation of clonogenic potential using CFU-GM assays.
  • Double processing of BM and BC for ABO-incompatible cases to reduce erythrocyte contamination.

    • Main Results:

    • Mean starting BM volume of 843 mL processed to a 230 mL BC in 6-8 cycles.
    • High MNC recovery of 92% with a mean starting count of 1.22 x 10^8/kg.
    • Successful reduction of donor erythrocytes by 98% in ABO-incompatible transplants through double processing.
    • CFU-GM assays confirmed adequate stem cell retention in the processed BC fraction.

    Conclusions:

    • The Haemonetics V50 cell separator provides an efficient method for bone marrow processing.
    • The buffy coat fraction obtained is suitable for cryopreservation and purging, retaining adequate stem cells.
    • The described processing method is effective in managing ABO-incompatible bone marrow transplants.