Investigations of red cell glyoxalase in recombinant families

The polymorphism of the red cell glyoxalase (GLO), which has recently been found to be linked with the major histocompatibility complex (HLA) in man, was investigated in a number of selected families showing various recombinations between HLA-A, HLA-B and the third locus of the phosphoglucomutase (PGM3). In two families with a recombination between HLA-A and HLA-B, the GLO allele travels with the HLA-B locus fragment of the chromosome, indicating that GLO is located on the side of the HLA-B locus. In other families, recombinations occurred between HLA-A and B on one side and GLO and PGM3 on the other side, demonstrating that GLO and PGM3 are located on the same side of HLA. Other recombinations separated PGM3 from GLO so that it can be assumed that GLO is located between HLA-B and PGM3. Thus the immunogenetic linkage group on human chromosome C6 has been increased by yet another outside marker gene to include now the following genes in this order: HLA-A--HLA-C--HLA-B--Bf--HLA-D--GLO--PGM3.