[Morphofunctional express-diagnosis of liver conditons]

Area of Science:

• Biomedical Engineering
• Histology
• Hepatology

Context:

• Assessing liver function is crucial for diagnosing and managing hepatic diseases.
• Current methods for evaluating liver status can be invasive or lack comprehensive real-time data.
• Developing efficient and simultaneous detection methods for hepatic indices is a significant challenge in clinical practice.

Purpose:

• To describe a novel method for the simultaneous detection of vital hepatic tissue indices.
• To utilize neutral red vital dye injected into the portal vein for comprehensive liver assessment.
• To establish a method for evaluating liver morphology, cellular absorption, microcirculation, and pH shifts.

Summary:

• The study details a method involving the injection of neutral red vital dye into the portal vein.
• Key hepatic indices assessed include liver tissue morphology, hepatic cell absorption capacity, blood microcirculatory efficacy, and pH shifts.
• The method involves histological preparation of liver slices and spectrophotometric quantification of extracted dye to estimate absorption.

Impact:

• This method offers a simultaneous and comprehensive approach to evaluating liver status.
• It provides valuable insights into liver tissue morphology, cellular function, and microcirculatory dynamics.
• The technique has potential applications in preclinical research and the development of diagnostic tools for liver diseases.