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Related Experiment Videos

Stem cell separation in paediatric patients.

J Zingsem1, V Weisbach, R Zimmermann

  • 1Department of Transfusion Medicine and Haemostaseology, Friedrich-Alexander Universität, Erlangen-Nümberg, Germany.

Transfusion Science
|November 3, 1996
PubMed
Summary
This summary is machine-generated.

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Peripheral blood stem cell (PBSC) apheresis was successfully adapted for pediatric patients using Cobe Spectra or Fresenius AS 104 separators. Procedures were modified for smaller patients, yielding comparable results to adults.

Area of Science:

  • Hematology
  • Pediatric Medicine
  • Biotechnology

Background:

  • Parenteral apheresis is crucial for stem cell collection.
  • Adapting apheresis for pediatric populations presents unique challenges.
  • Previous studies focused primarily on adult stem cell apheresis.

Purpose of the Study:

  • To evaluate the feasibility and efficacy of peripheral blood stem cell (PBSC) apheresis in pediatric patients.
  • To compare apheresis outcomes in children with those previously observed in adults.
  • To determine optimal procedural parameters for pediatric apheresis.

Main Methods:

  • 28 apheresis procedures were performed on 11 children (14-72 kg) using Cobe Spectra or Fresenius AS 104 cell separators.
  • Modified priming procedures (human albumin for <20 kg) and reduced blood flow rates (30-50 mL/min) were employed.

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  • Total blood volume processed was approximately three times the patient's blood volume.

    • Main Results:

    • Collected mononuclear cells (MNC) averaged 309.7 x 10^6.
    • Collected CD34+ cells averaged 2.01 x 10^6 per kg body weight.
    • Procedure duration ranged from 149 to 337 minutes.

    Conclusions:

    • PBSC apheresis can be successfully adapted for pediatric patients using standard cell separators.
    • Modified protocols ensure safety and efficacy in children of varying weights.
    • Apheresis outcomes in children are comparable to those achieved in adult populations.