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Proteomics: quantitative and physical mapping of cellular proteins.
1Biomolecular Structure Unit, Glaxo Wellcome Research and Development, Stevenage, UK. wpb0799@glaxowellcome.co.uk
Trends in Biotechnology
|April 6, 1999
Summary
Proteomics, the study of proteins, helps identify unknown gene functions. Techniques like 2D gel electrophoresis and mass spectrometry are key to understanding the proteome and advancing functional genomics.
Area of Science:
- Genomics
- Proteomics
- Functional Genomics
Background:
- Genome sequencing reveals many proteins with unknown functions.
- Identifying protein functions is crucial for understanding cellular processes.
Purpose of the Study:
- To highlight the role of proteomics in functional genomics.
- To describe methods for protein identification and functional analysis.
Main Methods:
- Two-dimensional gel electrophoresis.
- Mass spectrometry.
- Searches in protein and EST databases.
Main Results:
- These methods have significantly improved protein identification.
- Proteomics provides insights into the expressed protein complement of a genome.
Conclusions:
- Proteomics is essential for functional genomics.
- It can be divided into expression and cell-map proteomics for studying protein expression and interactions.