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Z/AP, a double reporter for cre-mediated recombination
C G Lobe1, K E Koop, W Kreppner
1Cancer Research Division, Sunnybrook Health Science Centre, 2075 Bayview Avenue, Toronto, Ontario, M4N 3M5, Canada.
Developmental Biology
|April 7, 1999
Summary
This study introduces a novel double-reporter mouse line for tracking Cre recombinase activity. This tool enables precise genetic manipulation in mammals, aiding research into development and disease.
Area of Science:
- Genetics
- Developmental Biology
- Mammalian Models
Background:
- The Cre/loxP system is crucial for genetic manipulation in mammals.
- Developing cell type-specific Cre-expressing lines is essential for targeted recombination.
- Existing methods for reporting Cre activity can be limited.
Purpose of the Study:
- To develop and characterize a double-reporter mouse line for reliable Cre recombinase activity readout.
- To provide a versatile tool for researchers utilizing the Cre/loxP system.
- To facilitate studies on mammalian development and disease.
Main Methods:
- Generation of a transgenic mouse line with a double-reporter system.
- Utilizing lacZ and human alkaline phosphatase genes as reporters.
- Characterization of reporter gene expression before and after Cre-mediated excision.
Main Results:
- The double-reporter line expresses lacZ before Cre excision.
- Cre-mediated excision leads to the expression of human alkaline phosphatase.
- Reporter activity is detectable across embryonic and adult stages and various lineages.
Conclusions:
- The developed double-reporter mouse line offers a convenient and reliable method to monitor Cre recombinase activity.
- This transgenic model is valuable for a wide range of applications using the Cre/loxP system.
- It will serve as an important reagent for genetic studies in mammals.