Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nucleus extraction from single mounted tissue sections.

T Liehr1, C Uwe, G Erich

  • 1Institute of Human Genetics, Jena, Germany. i8lith@mti-n.mti.uni-jena.de

Genetic Analysis : Biomolecular Engineering
|April 7, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Somatic homozygous loss of SH2B3, and a non-Robertsonian translocation t(15;21)(q25.3;q22.1) with NTRK3 rearrangement, in an adolescent with progenitor B-cell acute lymphoblastic leukemia with the iAMP21.

Cancer genetics·2022
Same author

A toddler with phylloid-type pigmentary mosaicism and ambiguous genitalia resulting from trisomy 14 induced by a der(Y)t(Y;14).

Human genome variation·2020
Same author

Chromosomal breakpoints in a cohort of head and neck squamous cell carcinoma patients.

Genomics·2019
Same author

BENIGN AND PATHOLOGICAL GAIN OR LOSS OF GENETIC MATERIAL — ABOUT MICROSCOPIC AND SUBMICROSCOPIC COPY NUMBER VARIATIONS (CNVs) IN HUMAN GENETICS.

Tsitologiia·2018
Same author

7q11.23 microduplication syndrome: neurophysiological and neuroradiological insights into a rare chromosomal disorder.

Journal of intellectual disability research : JIDR·2017
Same author

X-autosome and X-Y Translocations in Female Carriers: X-chromosome Inactivation Easily Detectable by 5-ethynyl-2-deoxyuridine (EdU).

Balkan journal of medical genetics : BJMG·2017
Same journal

Site-directed insertion and insertion-deletion mutations in the Escherichia coli chromosome simplified.

Genetic analysis : biomolecular engineering·1999
Same journal

DNA region responsible for transcriptional regulation of the Escherichia coli penicillin amidase (pac) gene by CRP and PAA.

Genetic analysis : biomolecular engineering·1999
Same journal

A reliable PCR amplification method for microdissected tumor cells obtained from paraffin-embedded tissue.

Genetic analysis : biomolecular engineering·1999
Same journal

Molecular genetic relationships between Bombycidae and Saturniidae based on the mitochondria DNA encoding of large and small rRNA.

Genetic analysis : biomolecular engineering·1999
Same journal

The gene for human transcription factor TCF11 is located telomeric to D17S1827, BTR and HP1Hsbeta on chromosome 17q22.

Genetic analysis : biomolecular engineering·1999
Same journal

Negative selection: a method for obtaining low-abundance cDNAs using high-density cDNA clone arrays.

Genetic analysis : biomolecular engineering·1999
See all related articles

A new method allows nuclei extraction from single mounted tissue sections for molecular cytogenetics. This technique enables fluorescence in situ hybridization (FISH) analysis on archival samples, crucial for cancer research.

Area of Science:

  • Molecular Biology
  • Genetics
  • Pathology

Background:

  • Archived formalin-fixed, paraffin-embedded (FFPE) tissues are valuable resources for molecular cytogenetic studies.
  • Traditional nuclear extraction methods often require unmounted tissue, limiting the use of valuable mounted archival samples.
  • Advancements in interphase cytogenetic analysis necessitate efficient methods for extracting nucleic acids from historical specimens.

Purpose of the Study:

  • To develop and validate a novel assay for extracting nuclei from a single mounted tissue section.
  • To assess the suitability of extracted nuclei for downstream molecular cytogenetic analyses, specifically fluorescence in situ hybridization (FISH).
  • To apply the developed method for investigating gene amplifications in oral cancer.

Main Methods:

Related Experiment Videos

  • A new assay was designed for the isolation of nuclei from a single mounted tissue section.
  • The extracted nuclei were utilized for fluorescence in situ hybridization (FISH) analysis.
  • The method was applied to analyze the INT2/FGF3-amplicon in 20 oral squamous cell carcinoma samples.

Main Results:

  • The developed assay successfully extracted interphase nuclei from single mounted tissue sections.
  • The extracted nuclei proved highly suitable for fluorescence in situ hybridization (FISH) analysis.
  • The INT2/FGF3-amplicon was successfully analyzed in 20 oral squamous cell carcinoma samples using this method.

Conclusions:

  • This novel nuclear extraction assay provides a valuable tool for molecular cytogenetic studies using mounted archival tissue.
  • The method facilitates the application of FISH analysis on FFPE samples, expanding research possibilities.
  • The successful analysis of the INT2/FGF3-amplicon in oral cancer highlights the clinical relevance of this technique.