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Application of bis-eugenol to a zinc oxide eugenol cement
S Fujisawa1, Y Kashiwagi, T Atsumi
1Department of Oral Diagnosis, Meikai University, School of Dentistry, Saitama-ken, Japan. fujisawa@dent.meikai.ac.jp
Objectives:
To assess the usefulness of dimerized eugenol (bis-eugenol) in dentistry, the physical properties of zinc oxide eugenol cement (ZOE) with bis-eugenol and the cytotoxicity of bis-eugenol were studied.
Methods:
Setting time, compressive strength, solubility and disintegration of ZOE cement with bis-eugenol according to the specifications of JDMAS315 were evaluated. The cytotoxicity of bis-eugenol and eugenol toward two different cell types, HGF (a primary culture of human gingival fibroblast) and HSG (a human epidermoid carcinoma cell line derived from a salivary gland) was evaluated by the MTT test and in terms of cell survival.
Results:
Addition of bis-eugenol to ZOE did not decrease the physical properties when employed at the ratio of 9:1 or 6:1 (liquid ND:bis-eugenol, w/w). Bis-eugenol was less toxic than eugenol in the cell culture tests.
Conclusions:
The results of this assay demonstrated that bis-eugenol is useful in ZOE.