Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Replicate and technician variation associated with computer aided bull sperm head morphometry analysis (ASMA).

C G Gravance1, D L Garner, C Pitt

  • 1Department of Obstetric and Gynaecology, University of Auckland, New Zealand.

International Journal of Andrology
|April 9, 1999
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prioritising in a crisis: what next for malaria research?

Malaria journal·2026
Same author

Small ruminant SexedULTRA™ sperm sex-sorting: Status report and recent developments.

Theriogenology·2021
Same author

Detection of bacterial pathogens in the hands of rural school children across different age groups and emphasizing the importance of hand wash.

Journal of preventive medicine and hygiene·2019
Same author

Evaluation of SexedULTRA 4M™ sex-sorted semen in timed artificial insemination programs for mature beef cows.

Theriogenology·2018
Same author

Conception risk of beef cattle after fixed-time artificial insemination using either SexedUltra™ 4M sex-sorted semen or conventional semen.

Theriogenology·2018
Same author

In vitro sperm quality and DNA integrity of SexedULTRA<sup>™</sup> sex-sorted sperm compared to non-sorted bovine sperm.

Theriogenology·2018

Computer-aided sperm head morphometry (ASMA) offers a repeatable and objective method for assessing bull sperm dimensions. This analysis shows no significant variation between technicians or replications, simplifying fertility assessments.

Area of Science:

  • Veterinary reproduction andrology
  • Spermatozoa morphometry
  • Quantitative semen analysis

Background:

  • Bull fertility is linked to spermatozoon abnormalities, but manual analysis methods are subjective and variable.
  • Computer-aided sperm head morphometry (ASMA) offers a precise alternative for measuring sperm dimensions.
  • The reliability of ASMA concerning technician and replication variability in bulls requires assessment.

Purpose of the Study:

  • To determine inter- and intra-analysis and technician variation in computer-aided bull sperm head morphometry.
  • To validate ASMA as a repeatable and objective tool for bull sperm head dimension analysis.

Main Methods:

  • Semen from 10 bulls was stained, and 250 sperm head images per slide were analyzed three times by two technicians using ASMA.

Related Experiment Videos

  • Morphometric dimensions (area, perimeter, length, width, width/length) were assessed using GLM-ANOVA.
  • Coefficients of variation (CVs) were calculated to evaluate intra- and inter-analysis and technician variability.

    • Main Results:

    • No significant differences (p > 0.1) were found between technicians or among bulls for any sperm head morphometric dimension.
    • No significant differences (p > 0.1) were detected between replicates for sperm head dimensions for either technician.
    • Mean intra-analysis CVs ranged from 4.5% (length) to 6.9% (area), and mean inter-analysis CVs ranged from 1.7% (width/length) to 3.0% (area).

    Conclusions:

    • Computer-aided sperm head morphometry analysis (ASMA) is a repeatable and objective method for assessing bull sperm head dimensions.
    • No significant variation exists between technicians or across replications, indicating ASMA's reliability for bull fertility studies.
    • Replicate analyses are not necessary when using ASMA, streamlining the process of acquiring accurate morphometric data.