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Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR

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Small ruminant SexedULTRA™ sperm sex-sorting: Status report and recent developments.

C González-Marín¹, C E Góngora¹, J F Moreno¹

¹Sexing Technologies, 22575 State Hwy 6 S, Navasota, TX, 77868, USA.

|January 14, 2021

Summary

Flow cytometry sperm sex-sorting technology has advanced significantly, bridging the fertility gap and enabling pre-sexed offspring production. This review covers recent developments in applying this technology to small ruminants like goats, sheep, and deer.

Keywords:

Fertility Sex-sorted sperm SexedULTRA™Small ruminants

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Area of Science:

Animal Science
Reproductive Biology
Biotechnology

Background:

Flow cytometry sperm sex-sorting, based on DNA differences between X- and Y-chromosome bearing sperm, is an established technique.
This method has enabled the production of pre-sexed offspring across various species and achieved commercial success in cattle over two decades.
Recent technological advancements have improved sorting efficiency and post-thaw sperm quality, narrowing the fertility gap with conventional sperm.

Purpose of the Study:

To review key advances and recent developments in sperm sex-sorting technology.
To explore the application of sex-sorted sperm in small ruminant industries (caprine, ovine, cervine).
To highlight improvements bridging the fertility gap for sex-sorted sperm.

Main Methods:

Review of existing literature and technological advancements in flow cytometry sperm sex-sorting.
Analysis of improvements in sorting efficiency and post-thaw sperm quality.
Examination of current applications and testing in small ruminant species.

Main Results:

Significant improvements in flow cytometry sex-sorting technology have enhanced efficiency and sperm quality.
The fertility gap between sex-sorted and conventional sperm has been substantially reduced.
Sex-sorted sperm is increasingly being tested and adopted in small ruminant industries.

Conclusions:

Advances in sperm sex-sorting technology are making it a viable tool for small ruminant production.
Improved fertility rates enhance the potential for commercial application in caprine, ovine, and cervine species.
Continued development promises wider application and success in diverse livestock industries.