Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Axonal cytoskeleton changes in experimental optic neuritis.

B Zhu1, G R Moore, T J Zwimpfer

  • 1Department of Ophthalmology, Vancouver Hospital and Health Science Center, University of British Columbia, 2550 Willow Street, Vancouver, British Columbia, Canada. bing@ecc.ubc.ca

Brain Research
|April 10, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[The clinical efficacy of unilateral biportal endoscopy contralateral inclinatory approach in the treatment of lumbar foraminal stenosis].

Zhonghua wai ke za zhi [Chinese journal of surgery]·2026
Same author

[Nasal infection with Mycobacterium avium complex: a case report].

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2025
Same author

Effect of stump-preserving arthroscopic reconstruction or stump-eliminating arthroscopic reconstruction combined with exercise rehabilitation therapy on knee functional recovery in patients with anterior cruciate ligament injuries.

Annals of the Royal College of Surgeons of England·2025
Same author

[A case of thoracic aortic aneurysm after coronary artery bypass grafting was treated with integrative branch stent combined with extracorporeal fenestration technique and internal branch technique].

Zhonghua xin xue guan bing za zhi·2025
Same author

Quantum Electrodynamics in Strong Electromagnetic Fields: Substate Resolved Kα Transition Energies in Heliumlike Uranium.

Physical review letters·2025
Same author

Association of treatment methods and survival in esophageal neuroendocrine carcinoma population of Surveillance, Epidemiology, and End Results database: a retrospective cohort study.

Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus·2025
Same journal

IGFBP3 and UBE2C are associated with protein modification pathways and serve as prognostic markers in glioma.

Brain research·2026
Same journal

Targeting neurodevelopmental miR132-3p promotes neuroprotection and axon regeneration after optic nerve injury in mice.

Brain research·2026
Same journal

Variability in acoustic startle response and prepulse inhibition across adulthood in Fragile X messenger ribonucleoprotein 1 knockout mice.

Brain research·2026
Same journal

Transcriptome-guided modeling reveals insulin-related metabolic dysfunction in SCA3 mouse cerebellum.

Brain research·2026
Same journal

Intranasal stromal cell-derived factor-1α mitigates parkinsonian deficits via dual modulation of neuroinflammation and gut microbiota in MPTP-induced models.

Brain research·2026
Same journal

Emotions, the amygdala, and the right hemisphere.

Brain research·2026
See all related articles

Acute demyelination in the rat optic nerve triggers local axonal cytoskeleton changes, with inflammation exacerbating damage. Macrophages appear key in this process, highlighting potential therapeutic targets for multiple sclerosis (MS).

Area of Science:

  • Neuroscience
  • Immunology
  • Pathology

Background:

  • Axonal loss is a significant contributor to permanent disability in multiple sclerosis (MS).
  • Understanding axonal degeneration mechanisms in demyelinating diseases is crucial for developing effective MS therapies.
  • Reliable models for studying demyelination are limited, hindering detailed research.

Purpose of the Study:

  • To investigate the mechanisms of axonal degeneration following acute demyelination.
  • To establish a localized rat optic nerve model for studying demyelination.
  • To examine the role of cytoskeleton changes and inflammation in axonal damage.

Main Methods:

  • Induction of local demyelination in the rat optic nerve using anti-galactocerebroside (anti-Gal C) antibody and complement.

Related Experiment Videos

  • Analysis of neurofilament (NF) and microtubule (MT) disassembly via immunohistochemistry (IHC) and electron microscopy (EM).
  • Assessment of inflammatory cell infiltration, including T lymphocytes, macrophages, and astrocytes.

    • Main Results:

    • Microinjection successfully induced local demyelination with minimal mechanical damage.
    • Early (day 1) moderate disassembly of NFs and MTs observed in demyelinated axons, preceding significant inflammation.
    • Progressive NF/MT changes and increased inflammatory cell infiltration (predominantly macrophages) noted from days 3-7.
    • Ultrastructural analysis confirmed inflammatory and axonal damage patterns.

    Conclusions:

    • Acute demyelination itself can initiate axonal cytoskeleton damage.
    • Subsequent local inflammation, particularly macrophage activity, likely amplifies axonal degeneration and loss.
    • This model provides insights into MS-related axonal damage and suggests macrophages as therapeutic targets.