Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Two operons that encode FNR-like proteins in Lactococcus lactis.

D O Gostick1, H G Griffin, C A Shearman

  • 1Department of Molecular Biology and Biotechnology, University of Sheffield, UK.

Molecular Microbiology
|April 14, 1999
PubMed
Summary

Researchers identified two FNR-like proteins (FlpA and FlpB) in Lactococcus lactis, which regulate zinc uptake and oxidative stress responses. The double mutant showed altered zinc content and increased hydrogen peroxide sensitivity.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Imaging Results from a Direct Conversion X-ray Detector with TlBr and CMOS Pixel Array.

IEEE transactions on nuclear science·2026
Same author

TlBr Films for Direct Digital Radiography.

IEEE transactions on nuclear science·2025
Same author

Nurses' perceptions of clinical leadership in the emergency department: A qualitative descriptive study.

International emergency nursing·2025
Same author

Perioperative care of patients undergoing total hip arthroplasty.

BJA education·2024
Same author

Defining remission in childhood-onset lupus: PReS-endorsed consensus definitions by an international task force.

Clinical immunology (Orlando, Fla.)·2024
Same author

Evidence for the earliest structural use of wood at least 476,000 years ago.

Nature·2023

Area of Science:

  • Microbiology
  • Molecular Biology
  • Bacterial Physiology

Background:

  • Global regulatory circuits, such as those mediated by CRP and FNR in Escherichia coli, are crucial for understanding and redirecting microbial metabolism.
  • Lactococcus lactis is a key bacterium in food fermentation, and understanding its regulatory mechanisms can optimize fermentation products.

Purpose of the Study:

  • To identify global regulatory circuits in Lactococcus lactis analogous to CRP and FNR in E. coli.
  • To investigate the role of FNR-like proteins in regulating carbon metabolism and stress responses in L. lactis.

Main Methods:

  • Polymerase chain reaction (PCR) was used to identify genes encoding FNR-like proteins.
  • Transcript analysis and promoter analysis were performed to understand gene regulation.

Related Experiment Videos

  • Phenotypic analysis of single and double mutants, including zinc content, oxidative stress sensitivity, and 2D gel electrophoresis, was conducted.

    • Main Results:

    • Two genes, flpA and flpB, encoding FNR-like proteins (FlpA and FlpB), were identified in L. lactis.
    • FlpA and FlpB are part of paralogous operons potentially involved in cation ATPases and storage proteins.
    • The double mutant exhibited reduced intracellular zinc content, increased sensitivity to hydrogen peroxide, and altered polypeptide profiles, suggesting FlpA and FlpB regulate overlapping modulons involved in zinc uptake and oxidative stress.

    Conclusions:

    • FlpA and FlpB are novel FNR-like regulators in Lactococcus lactis.
    • These regulators play a role in managing cellular responses to metal ion and oxidative stress.
    • The findings provide a basis for redirecting carbon metabolism by understanding these regulatory circuits.