Related Experiment Video

Updated: Aug 18, 2026

Development of a Human Preclinical Model of Osteoclastogenesis from Peripheral Blood Monocytes Co-cultured with Breast Cancer Cell Lines
06:00

Development of a Human Preclinical Model of Osteoclastogenesis from Peripheral Blood Monocytes Co-cultured with Breast Cancer Cell Lines

Published on: September 13, 2017

Oncostatin M: development of a pleiotropic cytokine

J K Loy1, T J Davidson, K K Berry

  • 1Department of Experimental Pathology, Bristol-Myers Squibb, Princeton, New Jersey 08543, USA. loyj@bms.com

Toxicologic Pathology
|April 20, 1999
PubMed

Insights

Oncostatin M (OM), an IL-6 cytokine, reduces inflammation and lethal effects in animal models. Preclinical studies show reversible side effects, suggesting therapeutic potential for chronic inflammatory diseases.

Area of Science:

  • Immunology
  • Molecular Biology
  • Pharmacology

Background:

  • Oncostatin M (OM) is a cytokine within the interleukin-6 (IL-6) subfamily, mediating biological effects through JAK-STAT and MAP kinase pathways.
  • OM influences cytokine expression and protease production, impacting inflammatory responses.
  • OM exhibits anti-inflammatory properties, reducing tumor necrosis factor-alpha (TNF-alpha) and mitigating lethal effects of lipopolysaccharide (LPS) in mice.

Framework:

  • OM's mechanism involves binding to its receptor, initiating intracellular signaling cascades.
  • In vitro studies demonstrate OM's capacity to modulate cytokine profiles and protease activity.
  • In vivo studies utilized LPS-challenged mice and animal models of inflammatory diseases to assess OM's efficacy.

Implementation:

  • OM administration in LPS-challenged mice led to reduced serum TNF-alpha levels and improved survival rates.
  • Therapeutic efficacy was observed in animal models for inflammatory bowel disease, arthritis, and experimental autoimmune encephalomyelitis.
  • Preclinical safety evaluations involved subcutaneous administration in mice and monkeys at various dose levels.

Implications:

  • Observed immunomodulatory effects suggest OM's potential as a therapeutic agent for chronic inflammatory conditions.
  • Reversible adverse effects noted in preclinical safety studies, including dermal irritation and transient changes in blood parameters, inform dosage and administration strategies.
  • Further research into OM's therapeutic applications is warranted based on its demonstrated anti-inflammatory and immunomodulatory capabilities.

More Related Videos

Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform11:00

Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform

Published on: November 9, 2017

Identification of Functional Protein Regions Through Chimeric Protein Construction11:39

Identification of Functional Protein Regions Through Chimeric Protein Construction

Published on: January 8, 2019

Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...