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Non-aqueous enzymology approach for improvement of reagentless mediator-based glucose biosensor
L V Lukachova1, A A Karyakin, Y N Ivanova
1Faculty of Chemistry, M. V. Lomonosov Moscow State University, Russia.
The Analyst
|April 21, 1999
Abstract:
The formation of Nafion membranes containing glucose oxidase and dimethylferrocene as a mediator was optimized using a previously reported non-aqueous enzymology approach for biosensor development. Enzyme immobilization in Nafion membranes was carried out from water-organic mixtures with a high content of organic solvent. The mediator based reagentless glucose electrode was tested in a flow injection system. The response towards glucose addition was stable: the reproducibility during 50 assays exceeded 95%. The response was linear over the glucose concentration range 0.5-50 mM.