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A self-calibrating microelectrode chip for glucose sensing in whole sturgeon blood
Wen Li1, Feng Lu2, Xiaoxiao Zhang1
1Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Ministry of Education, Shandong Key Laboratory of Analytical Chemistry for Life Science and Intelligent Detection, College of Chemistry and Molecular Engineering, College of Biological Engineering, State Key Laboratory of Advanced Optical Polymer and Manufacturing Technology, Qingdao University of Science and Technology, Qingdao 266042, China. hunxuprof@outlook.com.
Abstract:
An analog-digital self-calibrating microelectrode chip is reported for enzymatic glucose sensing in whole sturgeon blood. A non-enzymatic reference working electrode records common-mode background contributions, enabling fouling-induced signal drift to be compensated for by differential subtraction, while partial least squares regression (PLSR) refines residual matrix effects, enabling 0.1-8.0 mM detection, a 0.08 mM LOD, and short-term in vivo glucose tracking.
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