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Chemiluminescence assay for kanamycin based on target recycling strategy
Liyuan Yu1, Xiaoqian Zhang1, Daobin Jin1
1Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, China.
Abstract:
A chemiluminescence (CL) sensing strategy for kanamycin residue detection in fish samples was established based on luminol-functionalized gold nanoparticles as CL nanoprobe materials combined with DNA hairpin structure and carboxyl-modified magnetic beads. Relying on nucleic acid amplification technology, the system can successfully realize the recycling of kanamycin, so that the biosensor can release a large number of luminol-functionalized gold nanoparticles with excellent CL performance even at a low residual levels of kanamycin. The biosensor strategy showed a good linear relationship with kanamycin in the range 0.09-130 nM, the detection limit was as low as 0.04 nM. This method proves the excellent performance of the sensing strategy and provides a low-cost and high-sensitivity CL analysis strategy for the detection of kanamycin and even other antibiotics.
