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Related Experiment Videos

Leukocyte-endothelial cell interactions - lessons from knockout mice.

A R Rosenkranz1, T N Mayadas

  • 1Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, Mass., USA.

Experimental Nephrology
|April 24, 1999
PubMed
Summary

Gene targeting created knockout mice, revealing surprising roles for leukocyte adhesion receptors in development, obesity, and glomerulonephritis pathogenesis through functional synergies.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Gene targeting enables the creation of mouse models deficient in specific genes.
  • Leukocyte adhesion receptors play critical roles in cell-cell interactions, particularly in leukocyte trafficking.
  • Previous studies highlighted the importance of these receptors in various physiological and pathological processes.

Purpose of the Study:

  • To review the technique of gene targeting for creating deficient mouse models.
  • To provide an overview of leukocyte adhesion receptors and their corresponding knockout mice.
  • To discuss the role of leukocyte adhesion receptors in experimental glomerulonephritis pathogenesis.

Main Methods:

  • Gene targeting technology for generating knockout mouse strains.

Related Experiment Videos

  • Review of existing literature on leukocyte adhesion receptors and their functions.
  • Analysis of experimental glomerulonephritis models utilizing knockout mice.

    • Main Results:

    • Leukocyte adhesion receptor knockout mice have provided insights into leukocyte-endothelium interactions.
    • Surprising roles for these receptors identified in development and pathologies such as obesity.
    • Evidence for functional synergies between different adhesion receptors has been established.
    • Studies in knockout mice have elucidated the involvement of leukocyte adhesion receptors in glomerulonephritis.

    Conclusions:

    • Gene targeting and knockout mouse models are powerful tools for studying leukocyte adhesion receptor function.
    • Leukocyte adhesion receptors are crucial in immune cell trafficking and have broader implications beyond inflammation.
    • Understanding these receptors is vital for deciphering the pathogenesis of kidney diseases like glomerulonephritis.