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Related Experiment Video

Updated: Jun 5, 2026

Analysis of Physiologic E-Selectin-Mediated Leukocyte Rolling on Microvascular Endothelium

Analysis of Physiologic E-Selectin-Mediated Leukocyte Rolling on Microvascular Endothelium

Published on: February 11, 2009

Gene knockout on P-selectin: Its biology and function.

¹Tanya N. Mayadas is at the Vascular Research Division, Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Trends in Cardiovascular Medicine

|January 15, 2011

Summary

Mice lacking P-selectin, a molecule on activated platelets and cells, help researchers understand inflammation. These P-selectin-deficient mice are key tools for studying leukocyte extravasation in cardiovascular disease.

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Area of Science:

Immunology and Cell Biology
Cardiovascular Research
Inflammation Studies

Background:

P-selectin is a leukocyte adhesion molecule expressed on activated platelets and endothelial cells.
P-selectin expression is observed at sites of active inflammation, particularly in cardiovascular disease.
Understanding P-selectin's role is crucial for inflammatory process research.

Purpose of the Study:

To review the generation and characterization of P-selectin-deficient mice.
To elucidate the role of P-selectin in leukocyte extravasation.
To analyze P-selectin's involvement in leukocyte-endothelial and leukocyte-platelet interactions.

Main Methods:

Gene targeting to create P-selectin-deficient mouse models.
Characterization of these genetically modified mouse lines.
Review of existing literature on P-selectin function in inflammation.

Main Results:

P-selectin-deficient mice serve as valuable tools for studying inflammatory mechanisms.
These models facilitate the investigation of leukocyte adhesion and extravasation.
Insights into leukocyte-endothelial and leukocyte-platelet interactions have been gained.

Conclusions:

P-selectin plays a significant role in mediating leukocyte recruitment during inflammation.
P-selectin-deficient mice are essential for dissecting inflammatory pathways.
Further research using these models can advance understanding of inflammatory diseases like cardiovascular disease.