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Related Experiment Videos

Isolation, sequencing and expression of RED, a novel human gene encoding an acidic-basic dipeptide repeat.

E Assier1, H Bouzinba-Segard, M C Stolzenberg

  • 1INSERM U396, Institut Biomédical des Cordeliers, 15 rue de l'Ecole de Médecine, 75006, Paris, France.

Gene
|April 27, 1999
PubMed
Summary

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Researchers identified a novel human gene, RED, and its mouse counterpart, MuRED, characterized by a unique arginine-glutamic acid/aspartic acid (RED) repeat. These genes localize to nuclear dots, suggesting a role in gene regulation.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • A novel human gene, RED, and its murine homologue, MuRED, were identified.
  • These genes are named for their distinctive arginine-glutamic acid/aspartic acid (RED) repeat sequence.
  • Both genes are expressed across various tissues and the human RED gene is mapped to chromosome 5q22-24.

Purpose of the Study:

  • To characterize the novel RED and MuRED genes.
  • To investigate the subcellular localization and functional domains of the RED protein.
  • To explore the potential role of RED in cellular processes.

Main Methods:

  • Gene cloning and sequencing of RED and MuRED.
  • Analysis of gene expression patterns and chromosomal mapping.

Related Experiment Videos

  • Fluorescent tagging and transfection of cells to study protein localization.
  • Site-directed mutagenesis to identify functional domains within the RED protein.

    • Main Results:

    • RED and MuRED share 98% amino acid identity and encode a 557-amino acid protein.
    • The RED protein localizes to distinct nuclear dots in transfected cells, independent of PML or Coilin.
    • Specific deletions in the amino terminus affect nuclear import and nuclear dot formation, indicating critical functional domains.

    Conclusions:

    • The RED protein's nuclear localization and specific domain requirements suggest a regulatory function.
    • RED may play a role in transcriptional regulation within the nucleus.
    • Further studies are warranted to elucidate the precise function of RED in cellular pathways.