Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A sensitive method for measuring haemoglobin in gastric contents.

M A Fisher, J N Hunt

    Digestion
    |January 1, 1976
    PubMed
    Summary

    A new, sensitive method accurately measures gastric haemoglobin levels by tracking optical density changes. This technique can detect small amounts of blood loss from the stomach lining.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Altered nerve excitability properties after stroke are potentially associated with reduced neuromuscular activation.

    Clinical neurophysiology : official journal of the International Federation of Clinical Neurophysiology·2020
    Same author

    Removal of tick controls for animals entering the UK.

    The Veterinary record·2011
    Same author

    Rapidly fatal neuropathies and an ALS clinical presentation.

    Acta neurologica Scandinavica·2011
    Same author

    Progress in patients with peptic ulceration treated for more that five years with poldine, including a double-blind study.

    British medical journal·2010
    Same author

    Gastric hypersecretion with duodenal ulceration.

    Lancet (London, England)·2010
    Same author

    A method of estimating peptic activity in gastric contents.

    The Biochemical journal·2010

    Area of Science:

    • Biochemistry
    • Clinical Chemistry
    • Gastroenterology

    Background:

    • Accurate measurement of gastric bleeding is crucial for diagnosing gastrointestinal disorders.
    • Existing methods may lack the sensitivity to detect minimal blood loss.

    Purpose of the Study:

    • To describe a sensitive and quantitative method for measuring haemoglobin in gastric contents.
    • To establish the method's capability in detecting physiologically relevant levels of blood loss.

    Main Methods:

    • Utilizes the reaction between orthotolidine and hydrogen peroxide, catalyzed by haemoglobin.
    • Measures the rate of change in optical density of the reaction mixture.
    • Quantifies haemoglobin concentration based on the reaction kinetics.

    Main Results:

    • The described method demonstrates high sensitivity for haemoglobin detection.
    • It can accurately measure the amount of blood normally lost from the gastric mucosa within a 10-minute period.

    Conclusions:

    • This orthotolidine-based assay provides a sensitive tool for quantifying gastric haemoglobin.
    • The method is suitable for detecting occult gastrointestinal bleeding, aiding in early diagnosis and management.

    Related Experiment Videos