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Updated: Oct 23, 2026

Generation of Marked and Markerless Mutants in Model Cyanobacterial Species
Published on: May 29, 2016
Pseudoazurin mediates periplasmic electron flow in a mutant strain of Paracoccus denitrificans lacking cytochrome
M Koutný1, I Kucera, R Tesarík
1Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic.
Abstract:
A periplasmic protein able to transfer electrons from cytoplasmic membrane to the periplasmic nitrite reductase (cytochrome cd1) has been purified from the anoxically grown cytochrome c550 mutant strain Pd2121 and shown to be pseudoazurin by several independent criteria (molecular mass, copper content, visible spectrum, N-terminal amino acid sequence). Under our assay conditions, the half-saturation of electron transport occurred at about 10 microM pseudoazurin; the reaction was retarded by increasing ionic strength.
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