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Modulation of alpha-tropomyosin expression by alpha-tocopherol in rat vascular smooth muscle cells
E Aratri1, S E Spycher, I Breyer
1Institut für Biochemie und Molekularbiologie, Universität Bern, Switzerland.
FEBS Letters
|April 28, 1999
Abstract:
The effect of alpha-tocopherol (vitamin E) on gene expression in rat vascular smooth muscle cells was studied by the differential display technique. One gene out of about 1000 genes analyzed, identified as alpha-tropomyosin, showed an increased transcription level caused by alpha-tocopherol treatment. Northern and Western blot analysis revealed a time-dependent transient up-regulation of the amount of mRNA (peak between 2 and 3 h) and protein (peak at 5 h) in alpha-tocopherol-treated cells. No effect was observed in cells treated with beta-tocopherol.