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Synthesis of an antisense oligonucleotide targeted against C-raf kinase: efficient oligonucleotide synthesis without
A H Krotz1, D L Cole, V T Ravikumar
1Isis Pharmaceuticals Inc., Carlsbad, CA 92008, USA.
Bioorganic & Medicinal Chemistry
|April 29, 1999
Abstract:
It is demonstrated that a solution of dichloroacetic acid in toluene removes dimethoxytrityl groups from the 5'-terminus of an antisense phosphorothioate oligodeoxyribonucleotide (ISIS 5132/CGP69846A) during synthesis on solid support cleanly and efficiently. It is therefore suggested to replace health hazardous dichloromethane which is typically used in oligonucleotide synthesis as solvent for DMTr-removal by toluene.