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Ximdisp--A visualization tool to aid structure determination from electron microscope images.

J M Smith1

  • 1MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, United Kingdom.

Journal of Structural Biology
|May 1, 1999
PubMed
Summary

Ximdisp is a versatile X-windows program for visualizing electron microscope images and density maps. It aids in macromolecular structure computation through interactive tools for particle selection and data processing.

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Area of Science:

  • Structural Biology
  • Biophysics
  • Computational Biology

Background:

  • Accurate visualization of digitized electron microscope images is fundamental for macromolecular structure determination.
  • Effective 3D rendering of computed density maps is essential for understanding molecular shapes.
  • Existing software may lack comprehensive interactive features for diverse image processing needs.

Purpose of the Study:

  • To introduce Ximdisp, a core component of the MRC image processing package, designed for interactive display and analysis of electron microscopy data.
  • To detail the functionalities of Ximdisp in various structural biology applications, including single-particle analysis, 2D crystallography, and helical structure analysis.
  • To demonstrate how Ximdisp facilitates manuscript illustration through versatile color representations and interactive controls.

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Main Methods:

  • Ximdisp utilizes an X-windows based, menu-driven interface for user-friendly operation.
  • The program supports the display of raw electron microscope images, Fourier transforms, and computed density maps.
  • Interactive features include particle selection, lattice refinement, filament straightening, and Fourier transform analysis.

Main Results:

  • Ximdisp provides interactive control over image display with clear menus and editable dialogue boxes.
  • It offers various display modes and color representations suitable for publication.
  • The software integrates seamlessly into workflows for single-particle analysis, 2D crystal processing, and helical structure analysis.

Conclusions:

  • Ximdisp is a powerful and flexible tool for visualizing and processing electron microscopy data in structural biology.
  • Its interactive nature and diverse functionalities enhance the efficiency of macromolecular structure computation.
  • The program effectively supports the entire workflow from raw image display to final density map analysis and manuscript preparation.