Active and passive characteristics of the canine cricothyroid muscles

Active and passive characteristics of the canine cricothyroid muscle were investigated through a series of experiments conducted in vitro and compared with their counterparts in the thyroarytenoid muscle. Samples from separate portions of canine cricothyroid muscle, namely, the pars recta and pars obliqua, were dissected from dog larynges excised a few minutes before death and kept in Krebs-Ringer solution at a temperature of 37 degrees C +/- 1 degrees C and a pH of 7.4+/-0.05. Active tetanic stress was obtained in isometric and isotonic conditions by applying field stimulation to the muscle samples through a pair of parallel-plate platinum electrodes and using a train of square pulses of 0.1-ms duration and 85-V amplitude. Force and elongation of the samples were obtained electronically with a dual-servo system (ergometer). The results indicate that the dynamic response of the canine cricothyroid muscle is almost twice as slow as that of the thyroarytenoid muscle. The average 50% tetanic contraction times for pars recta and pars obliqua were 84 ms and 109 ms, respectively, in comparison to 50 ms for thyroarytenoid. The examination of force-velocity response of this muscle indicates a maximum shortening velocity of 2 to 3 times its length per second, which is about half of the thyroarytenoid shortening speed. The passive properties of the pars recta and pars obliqua portions are similar to those of thyroarytenoid muscle.