Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Effect of factor XIII on endothelial barrier function.

T Noll1, G Wozniak, K McCarson

  • 1Physiologisches Institut, Justus-Liebig-Universität, D-35392 Giessen, Germany. thomas.noll@physiologie.med.uni-giessen.de

The Journal of Experimental Medicine
|May 4, 1999
PubMed
Summary

Activated factor XIII significantly reduces endothelial permeability and preserves barrier function during metabolic stress. This finding highlights factor XIII

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Non-invasive methods for diagnosing pregnancy in cows and their real value.

Polish journal of veterinary sciences·2024
Same author

Large-aperture coatings for fusion-class laser systems.

Applied optics·2020
Same author

[Point-of-care ultrasonography of the abdomen in emergency and intensive care medicine].

Medizinische Klinik, Intensivmedizin und Notfallmedizin·2018
Same author

Development of a hard X-ray split-and-delay line and performance simulations for two-color pump-probe experiments at the European XFEL.

The Review of scientific instruments·2018
Same author

International comparative field study evaluating the assay performance of AFSTYLA in plasma samples at clinical hemostasis laboratories.

Journal of thrombosis and haemostasis : JTH·2017
Same author

Ultrahypofractionated CyberKnifeTM based stereotactic radiotherapy versus conventional radiotherapy in patients with prostate cancer - acute toxicity evaluation in two phase II prospective studies.

Neoplasma·2017

Area of Science:

  • Vascular Biology
  • Biochemistry
  • Cell Biology

Background:

  • Endothelial barrier integrity is crucial for regulating vascular permeability.
  • Factor XIII (FXIII) is a transglutaminase involved in hemostasis.
  • The role of FXIII in endothelial barrier function is not fully understood.

Purpose of the Study:

  • To investigate the effect of activated factor XIII on endothelial barrier function.
  • To determine the specific subunit of factor XIII responsible for this effect.
  • To assess the protective role of activated factor XIII against hyperpermeability.

Main Methods:

  • Cultured porcine aortic endothelial cell monolayers.
  • Saline-perfused rat hearts model.
  • Measurement of albumin permeability.

Related Experiment Videos

  • Immunoreactive deposition analysis.
  • Induction of hyperpermeability via metabolic inhibition.

    • Main Results:

    • Thrombin-activated factor XIII reduced endothelial monolayer albumin permeability by 30%.
    • The activated A subunit of factor XIII mediated this permeability reduction.
    • Activated factor XIII A localized at intercellular junctions.
    • Activated factor XIII prevented hyperpermeability induced by metabolic inhibition.
    • Activated factor XIII reduced myocardial water content in ischemic-reperfused hearts.

    Conclusions:

    • Activated factor XIII significantly reduces endothelial permeability.
    • The activated A subunit of factor XIII is responsible for modulating endothelial barrier function.
    • Activated factor XIII protects the endothelial barrier during energy depletion.
    • FXIII's effect may involve modification of paracellular pathways.