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Eosinophil development and bone marrow and tissue eosinophils in atopic asthma
D S Robinson1, J North, K Zeibecoglou
1Allergy and Clinical Immunology, Imperial College School of Medicine, National Heart and Lung Institute, London, UK. d.s.robinson@ic.ac.uk
International Archives of Allergy and Immunology
|May 4, 1999
Summary
Interleukin-5 (IL-5) and eotaxin influence eosinophil development from bone marrow (BM) progenitors. Asthmatic BM shows increased eosinophils and early receptor acquisition, suggesting IL-5 acts early and eotaxin mobilizes BM eosinophils.
Area of Science:
- Immunology
- Hematology
- Respiratory Medicine
Background:
- Eosinophils originate from bone marrow (BM) progenitors.
- Interleukin-5 (IL-5) and eotaxin are implicated in eosinophil expansion and mobilization in asthma.
Purpose of the Study:
- To investigate phenotypic changes during eosinophil lineage development from CD34+ cells in vitro.
- To assess the expression of the eotaxin receptor, CCR3, on BM eosinophils from asthmatic and control subjects.
Main Methods:
- Examined phenotypic changes of CD34+ cells differentiating into eosinophils in vitro.
- Evaluated BM eosinophils from asthmatic and control individuals for CCR3 expression.
Main Results:
- Receptors for IL-5 and CCR3 were acquired early in eosinophil development.
- Increased numbers of CD34+ cells, mature, and immature CCR3+ eosinophils were observed in BM from asthmatic patients.
Conclusions:
- IL-5 appears to play a role early in eosinophil development.
- Eotaxin demonstrates potential to mobilize a BM eosinophil pool in asthma.