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Alteration in diastolic function following cardiac cryopreservation at subzero temperatures
E Gutiérrez-Carretero1, R Bello-Puentes, J M Borrego-Domínguez
1Virgen del Rocio Hospital, Seville, Spain.
Summary
Cryopreservation of rabbit hearts at subzero temperatures significantly improved systolic function but worsened diastolic function. This involved increased stiffness and altered pressure-volume curves, impacting cardiac performance.
Area of Science:
- Cardiology
- Cryobiology
- Physiology
Background:
- Investigating the effects of cryopreservation on cardiac function is crucial for organ preservation.
- Subzero temperature protocols are employed to maintain tissue viability.
Purpose of the Study:
- To evaluate the impact of cryopreservation on left ventricular diastolic function.
- To assess changes in cardiac mechanics following cryopreservation.
Main Methods:
- Utilized isolated rabbit hearts for the study.
- Employed a cryopreservation protocol using 5% polyethylene glycol (PM 4000) as a cryoprotective agent.
Main Results:
- Observed a statistically significant improvement in systolic function post-cryopreservation.
- Demonstrated a significant worsening of diastolic function (p < 0.05).
- Noted an increase in mean stiffness and a decrease in differential stiffness (p < 0.05).
Conclusions:
- Cryopreservation at subzero temperatures negatively affects diastolic function.
- Changes in stiffness and pressure-volume relationships indicate impaired diastolic mechanics.
- Further research is needed to optimize cryopreservation techniques for preserving cardiac diastolic function.