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Sexual Crosses with the Mucoromycete Phycomyces blakesleeanus
Published on: June 6, 2025
In Vitro Sexual Reproduction of Botrytis cinerea
1Wageningen University, Laboratory of Phytopathology, Droevendaalsesteeg 1, 6708PB, Wageningen, The Netherlands.
Methods in Molecular Biology (Clifton, N.J.)
|September 26, 2026
Abstract:
Sexual reproduction is a crucial aspect of the lifecycle of Botrytis cinerea. This protocol outlines a method to induce sexual reproduction of B. cinerea under laboratory conditions and obtain ascospores from which sexual offspring can be grown. A video was prepared that illustrates the entire process.

