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Brachyury regulatory region active in embryonal carcinoma P19 cells
H Yamaguchi1, Y Kitagawa, K Miki
1Graduate Program for Biochemical Regulation, Graduate School of Bioagricultural Sciences, Nagoya University, Japan.
Bioscience, Biotechnology, and Biochemistry
|May 5, 1999
Abstract:
Brachyury (T) is involved in mesoderm induction during early mouse development. We analyzed the region regulating expression of T in embryonal carcinoma P19 cells, which differentiate into mesoderm derivatives in vitro. Transfection of plasmids encoding reporter genes under the control of the 5'-flanking region showed positive regulatory elements between -298 and -129 bp are responsible for driving T expression in mesodermal cells.