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Updated: Jul 22, 2026

Murine Model of Allergen Induced Asthma
Published on: May 14, 2012
Large porous particles for sustained protection from carbachol-induced bronchoconstriction in guinea pigs
A Ben-Jebria1, D Chen, M L Eskew
1Department of Chemical Engineering, The Pennsylvania State University, University Park 16802, USA. axb23@psu.edu
Purpose:
To determine whether a new formulated albuterol aerosol could sustain inhibition to bronchoconstriction for approximately one day in guinea pigs challenged with carbachol.
Methods:
Large and porous particles, comprising a combination of endogenous or FDA-approved excipients and albuterol sulfate, were prepared by spray drying using a NIRO portable spray drier. The anesthetized animals inhaled 5 mg of large porous or small nonporous particles by forced ventilation via cannulae inserted in the lumen of their exposed tracheae. At regular intervals over a period of 36 hours after drug delivery, airway resistance was determined in response to carbachol challenge dose.
Results:
Whereas inhalation of small nonporous albuterol particles protected from the carbachol-induced bronchoconstriction for up to 5 hours, inhalation of large porous albuterol particles produced a significant inhibition of carbachol-induced bronchoconstriction for at least 16 hours.
Conclusions:
The absence of substantial side effects, verified over a period of 24 hours by evaluating cardio-respiratory parameters as well as pulmonary inflammation, supports the utility of large porous albuterol particles for sustained therapies in asthma and other types of lung disease.
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