Activation and regulation of the IkappaB kinase in human B cells by CD40 signaling
Related Experiment Video
Updated: Jul 17, 2026

Generation of Human CD40-activated B cells
Published on: October 16, 2009
Y Kosaka1, D M Calderhead, E M Manning
1Graduate Program in Molecular and Cellular Biology, Dartmouth Medical School, Lebanon, NH 03756, USA.
Abstract:
This study demonstrates that the engagement of CD40 results in the activation of the recently described IkappaB kinase (IKK) in a human B cell line. The kinase appears to reside within the cell in a cytosolic signalsome complex consisting of IKK, IkappaB, and an MKP-1-like molecule. While the binding of CD154 to CD40 induces the assembly of a CD40-TRAF receptor complex, IKK is not recruited to this complex. Nonetheless, a functional link between TRAF2 and IKK activity in B cells is demonstrated by the fact that overexpression of TRAF2 constitutively induces IKK activity, NF-kappaB luciferase and Fas expression. Synergy in the activation of IKK and NF-kappaB-dependent gene expression was observed by the simultaneous engagement of the B cell receptor and CD40, establishing an early means for cross-talk between these two B cell activation pathways. This study discusses the sequential biochemical events that transpire upon CD40 engagement by its ligand in human B cells.
Related Concept Videos
Positive Regulator Molecules
Inhibition of Cdk Activity
MAPK Signaling Cascades
The JAK-STAT Signaling Pathway
PI3K/mTOR/AKT Signaling Pathway
Inhibition of CDK Activity

