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Updated: Oct 23, 2026

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy
Published on: November 11, 2025
Effect of spatial inhomogeneity in dielectric permittivity on DNA double layer formation
1Department of Biochemistry, University of Minnesota, St. Paul, Minnesota 55108, USA. j.maarel@rulgca.leidenuniv.nl
Abstract:
In solutions of tetramethylammonium (TMA+) DNA (double stranded) without added low-molecular-weight salt, the counterion radial density is calculated using the cylindrical Poisson-Boltzmann equation with a distance-dependent quasimacroscopic dielectric permittivity. Comparisons with small-angle neutron scattering data indicate that any inhomogeneity in dielectric permittivity is confined to one or two solvent layers from the DNA surface. At least for TMA+, which may be too large to penetrate the grooves of DNA to any significant extent, dielectric inhomogeneity modeled in this way has no detectable effect on the radial distribution.
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