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[Use of rapid methods in detection of enteroviruses]
Klinicheskaia Laboratornaia Diagnostika
|May 11, 1999
Abstract:
Indirect immunofluorescence (IIF) and RT-PCR were used for rapid diagnosis of enterovirus infection during an outbreak of aseptic meningitis in Gomel. IgM to enterovirus were detected in 93.6% sera by IIF and in 71.4% cerebrospinal fluid specimens by RT-PCR. IIF takes only 1.5-2 h and RT-PCR 6-7 h. The methods are recommended for rapid diagnosis of enterovirus infection.
Insights
Rapid diagnosis of enterovirus infection is crucial. Indirect immunofluorescence (IIF) and RT-PCR offer quick detection of IgM to enterovirus, aiding in aseptic meningitis outbreak management.
Area of Science:
- Virology
- Immunology
- Infectious Diseases
Background:
- Enterovirus infections can cause aseptic meningitis.
- Rapid diagnostic methods are essential during outbreaks.
Purpose of the Study:
- To evaluate indirect immunofluorescence (IIF) and RT-PCR for rapid enterovirus diagnosis.
- To compare the efficacy and speed of IIF and RT-PCR.
Main Methods:
- Indirect immunofluorescence (IIF) assay for IgM detection.
- Reverse transcription-polymerase chain reaction (RT-PCR) for enterovirus RNA detection.
Main Results:
- IIF detected IgM to enterovirus in 93.6% of sera.
- RT-PCR detected enterovirus in 71.4% of cerebrospinal fluid specimens.
- IIF turnaround time: 1.5-2 hours; RT-PCR turnaround time: 6-7 hours.
Conclusions:
- Both IIF and RT-PCR are effective for rapid enterovirus diagnosis.
- IIF is faster than RT-PCR for IgM detection.
- These methods are recommended for managing aseptic meningitis outbreaks.