[Automatic ultramicrotechnic for the determination of plasma uric acid]

An automatic micromethod is described for the estimation of plasma uric acid. The hydrogen peroxide formed when uric acid is oxidised by uricase, condenses by oxidation two molecules of p-hydroxyphenil-acetic acid in the presence of peroxidase. The product formed (dicarboxymethyl-5,5' dihydroxy-2,2' biphenyl) is fluorescent. This method permits one to estimate quantitites of uric acid of the order of 1 microng and permits measurement of uric acid in 50 microll of plasma. The values obtained for human plasma are compared to those supplied by a reductrimetric technique (SMA 12-60).