[Method of gradient chromatography for the isolation of purified lipid fractions from nerve tissue in studies using...

A method is developed for separation of lipids from nervous tissue by thin-layer chromatography in silica gel. Cholesterol and "minor" lipids were removed from the start by the first chromatography in the system ether-benzol-ethanol-acetic acid. Phospholipids and galactolipids, which remained at the start, were separated by the repeated chromatography in the system chloroform-methanol-NH4OH. The purified fractions of cholesterol, cerebrosides, sulfatides and the total fraction of phospholipids were obtained from 100-150 mg of nervous tissue in amounts, sufficient for estimation of their specific radioactivity in experiments with 14C.