An electron microscope cytochemical study of calcium ion localization in the honey-bee flight muscle

The authors studied the sarcomere ultrastructure and distribution of calcium ions in the cross striated flight muscles (longitudinal-dorsal and tergosternal) of the honey bee after being fixed in osmium tetraoxide and buffered to 7.4 pH and also according to the method of Carasso and Favard (1966). they ascertained an intensive and constant reaction to the presence of calcium on the level of myofilaments within the periphery of the sarcomeres and light and dark mitochondria. Calcium concentrations are not detectable in the Z line and M line. The authors carried out a control of the results using the method of Carasso and Favard (1966).